Sharp PC-9300 Technické informace stáhnout pdf (Strana 57)

9 Troubleshooting

www.turnerbiosystems.com 52

9.2 Table of Common Problems

Symptom

Possible Cause

Resolution

Injections sputter,

drip, or are

otherwise weak

Air bubbles are blocking the

line

• Make sure the stainless steel tubes are

completely inserted to the bottom of

the reagent container

Reagent residue is clogging

the tip

• Soak the injector tip in warm deionized

water and wipe it clean

Injector leaks.

Injector tips are damaged or

bent

• See Section 1 for instructions on

replacing the injector tip

The inlet or outlet tubing is not

properly connected to the

injector syringe

• See Section 1 for instructions on

removing or replacing the tubing

assembly

Injector tips do

not sit properly in

the injector tip

holder

Reagent residue has built up

inside the injector tip holder

• Clean the holder with 70% ethanol and

a damp cloth

Injectors not

injecting

Air bubbles are blocking the

line

• FLUSH the injectors to remove any air

bubbles

The end of the stainless steel

tube is not in the reagent

• Make sure the stainless steel tube is

completely inserted to the bottom of

the reagent container

• Re-prime the injectors

Reagent residue is clogging

the tubing

• Replace tubing, if needed

• Always flush the injector system after

use to prevent build up of reagent

residue

Reagent residue is clogging

the valves

• Contact technical support

Wetness appears

on the top of the

sample tray cover

after a run

Injector tips are bent

•

Clean up spill and replace injector tips

Microplate has overflowed

inside instrument

• Clean the optical head

• Check that the total volume in each

well is less than 250ul

• Thoroughly clean the interior of the

instrument

Reagent spilled inside the

instrument

• Wipe up spills immediately using 70%

ethanol and a damp cloth

Standard Light

Plate reads very

low or blank

The Standard Light Plate was

not turned on before the run

• Touch the START button on the

Standard Light Plate

• It will automatically turn off after 5

minutes

The selected wells do not

correlate with the Standard

Light Plate

• The well locations on the Standard

Light Plate are B2, D2, and F2

The Standard Light Plate was

placed in the wrong orientation

• Make sure the A1 notch on the

Standard Light Plate is in the upper

right corner

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